Cryo-EM V-Kit

Vitrification Starter Kit

Catálogo Nº Apresentação Preço (R$) Comprar / Observação
X-CEM-301 1 Kit Sob demanda Adicionar ao Carrinho

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C / ambient temperature

Validade: 12 months

Formulários:
Optimization of the vitrification process in cryo-EM sample preparation

Descrição:
The Cryo-EM V-Kit contains 10 different surfactants plus glycerol as cryoprotectant and 4 different types of Quantifoil grids. It is designed as a Vitrification Starter Kit to facilitate the search for the optimum vitrification condition and cryo-EM grid for both soluble and membrane proteins.

Contente:
Surfactants and Cryoprotectants || Amount || Conc. || CMC || Class Fluorinated Octyl Maltoside (FOM) || 100 μl || 0.41% (w/v) || 0.07% (w/v) || non-ionic detergent Hexadecyl-trimethyl-ammonium Bromide (CTAB) || 100 μl || 0.34% (w/v) || 0.03% (w/v) || cationic detergent n-Decyl-ß-D-Maltoside (DM) || 100 μl || 0.87% (w/v) || 0.09% (w/v) || non-ionic detergent n-Decyl-α-D-Maltoside (DαM) || 100 μl || 0.46% (w/v) || 0.08% (w/v) || non-ionic detergent n-Dodecyl-ß-D-Maltoside (DDM) || 100 μl || 0.09% (w/v) || 0.01% (w/v) || non-ionic detergent Sodium Deoxycholate || 100 μl || 1.66% (w/v) || 0.17% (w/v) || anionic detergent Triton X-100 || 100 μl || 0.15% (w/v) || 0.01% (w/v) || non-ionic detergent Tween 20 || 100 μl || 1% (w/v) || 0.01% (w/v) || non-ionic detergent CHAPSO || 100 μl || 2.5% (w/v) || 0.5% (w/v) || zwitterionic detergent Amphipol A8-35 || 100 μl || 5% (w/v) || || anionic surfactant Glycerol || 1 ml || 30% (w/v) || || cryoprotectant Quantifoil Holey Carbon Films 20x Quantifoil™ R 1.2/1.3 on 200 copper mesh 20x Quantifoil™ R 1.2/1.3 with ultrathin (2 nm) continuous layer of carbon on top on 200 copper mesh 20x Quantifoil™ R 2/1 on 300 copper mesh 20x Quantifoil™ R 2/1 with ultrathin (2 nm) continuous layer of carbon on top on 300 copper mesh

Referências selecionadas:
[1] Noble et al. (2018) Routine Single Particle CryoEM Sample and Grid Characterization by Tomography. DOI: 10.7554/eLife.34257.
[2] Thonghin et al. (2018) Cryo-electron microscopy of membrane proteins. Methods 147:176.
[3] Drulyte et al. (2018) Approaches to altering particle distributions in cryo-electron microscopy sample preparation. Acta Cryst. D 74:560.
[4] Glaeser et al. (2017) Opinion: hazards faced by macromolecules when confined to thin aqueous films. Biophys Rep 3:1.
[5] Gatsogiannis et al. (2016). Membrane insertion of a Tc toxin in near-atomic detail. Nat. Struct. Mol. Biol. 23:884.
[6] Efremov et al. (2015) Architecture and conformational switch mechanism of the ryanodine receptor. Nature 517:39.