Exo + SAP

Mix de Exonuclease/Fosfatase Alcalina para clean-up de produtos de PCR

Catálogo Nº Apresentação Preço (R$) Comprar
DPK-100XS 40 μL171,60 Adicionar ao Carrinho
DPK-100S 200 μL566,90 Adicionar ao Carrinho
DPK-100M 500 μL1.414,40 Adicionar ao Carrinho
DPK-100L 1 mL2.288,00 Adicionar ao Carrinho
DPK-100XL 4 x 1 mL8.548,80 Adicionar ao Carrinho

For in vitro use only!

Formulários:
Exo + SAP Kit is a quick, easy, and reliable enzymatic cleanup reagent for PCR product cleanup.
1. Removes excess primers and dNTPs
2. Fast 20 minutes protocol
3. Add directly to PCR product
4. 100 % Sample Recovery
5. Scalable for different reaction sizes
6. No interference on downstream applications
7. Easy to automate

Descrição:
The Kit contains two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I). The combination of these enzymes ensures complete dephosphorylation of dNTPs and degradation of residual primers, enabling downstream applications, such as sequencing, genotyping, cloning or SNP analysis. The reagents are active in commonly used PCR buffers and eliminates the need for additional buffer exchange.

Contente:
Exo + SAP mix

Protocol:

Add 2 μl of Exo + SAP mix directly to 5 μl PCR product (or scale up, e.g. 4 μl SAP-Exo mix to 10 μl PCR product) and mix well;

Incubate for 10 minutes at 37 °C;

Inactivate for 10 minutes at 80 °C.

PCR products are ready for downstream applications without any need for further processing.