Klentaq Pol Hot Start
DNA polimerase ativada por temperatura, modificada quimicamente
Thermus aquaticus, recombinante, E. coli
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| POL-128XS | 100 U | 900,94 | Adicionar ao Carrinho |
| POL-128S | 250 U | 1.774,77 | Adicionar ao Carrinho |
| POL-128M | 500 U | 3.374,34 | Adicionar ao Carrinho |
| POL-128L | 1.000 U | 6.266,19 | Adicionar ao Carrinho |
| POL-128XL | 2 x 1.000 U | 10.593,79 | Adicionar ao Carrinho |
Definição de unidade: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTPs into an acidinsoluble form in 30 minutes at 74 °C.
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Validade: 12 months
Concentração: 5 units/μL
Descrição:
Klentaq Pol Hot Start provides improved specificity and sensitivity when amplifying low copy-number targets in complex backgrounds or when prolonged room-temperature set up is required. This ultra-pure enzyme, in addition to its hot start capabilities, reduces false positives, amplifies a wide range of DNA sequence contexts. Klentaq Pol is purified by an additional separation process to reduce contaminating bacterial DNA sequences from the enzyme preparation The polymerase activity is chemically blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup. The enzyme catalyzes the polymerization of nucleoside into duplex DNA in 5’3’ direction in the presence of magnesium. Klentaq lacks the 5’-3’ exonuclease activity.
Contente:
Klentaq Polymerase Hot Start(blue cap)
5 units/μl Klentaq Pol Hot Start in Tris-HCl pH 9.0 (25 °C), KCl, EDTA, 50% (v/v) Glycerol and stabilizers.
Klentaq Pol HS Reaction Buffer complete (red cap) - 10x conc.
Tris-HCl pH 8.0 (25°C) and stabilizers.