Nucleodídeos para Uso em Epigenética
Table 1: Selection guide for epigenetic modification analysis.
| Epigenetic modification | Nucleotides and Nucleosides | References |
|---|---|---|
| DNA Methylation | N6-Methyl-dATP | |
| 5-Carboxy-2'-dC | ||
| 5-Formyl-2'-dC | ||
| 5-Methyl-2'-dC | ||
| 5-Hydroxymethyl-2'-dC | ||
| 5-Aza-dCTP | ||
| 5-Methyl-dCTP | [3,4] | |
| 5-Hydroxymethyl-dCTP | ||
| RNA Methylation | N6-Methyl-ATP | [2] |
| 2'OMe-ATP | [2] | |
| 5-Methyl-CTP | [5] | |
| 5-Hydroxy-UTP | ||
| 4SedTTP | [6] |
Produtos
Selected References
[1] Portela et al. (2010) Epigenetic modifications and human disease. Nature Biotechnology 28 (10):1057.
[2] Meyer et al. (2012) Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3' UTRs and near Stop Codons. Cell 149 (7):1635.
[3] Frauer et al. (2009) A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding. Nucleic Acids Research 37 (3):e22.
[4] Kaito et al. (2001) Activation of the maternally preset program of apoptosis by microinjection of 5-aza-2'-deoxycytidine and 5-methyl-2'-deoxycytidine-5'-triphosphate in Xenopus laevis embryos. Development, Growth & Differentiation 43 (4):383.
[5] Karikó et al. (2005) Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modification and the Evolutionary Origin of RNA. Immunity 23:165.
[6] Hong et al. (2018) Precise Antibody-Independent m6A Identification via 4SedTTP-Involved and FTO-Assisted Strategy at Single-Nucleotide Resolution. J. Am. Chem. Soc. 140 (18):5886.