Bst Polymerase 2.0 Turbo
Bst polimerase para amplificação isotérmica LAMP
| Catálogo Nº | Apresentação | Preço (R$) | Comprar |
|---|---|---|---|
| POL-134XS | 500 U | 210,00 | Adicionar ao Carrinho |
| POL-134S | 2.000 U | 670,32 | Adicionar ao Carrinho |
| POL-134L | 10.000 U | 2.680,65 | Adicionar ao Carrinho |
Figure 1:
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Validade: 12 months
Concentração: 8 units/μL
Descrição:
Bst 2.0 Turbo Polymerase is a genetically enhanced Bst 2.0 polymerase of the next generation. The polymerase is the ideal choice for ultra-fast and robust amplification of DNA at constant temperature (60 to 65 °C). The enzyme shows high strand displacement activity and generates an amplification factor of up 9 to 10 which is comparable to approx. 30 cycles in a PCR assay. The polymerase is 2-3 x faster compared to convencional Bst Polymerase and allows detection of a target gene within 10-30 minutes.
Contente:
Bst 2.0 Turbo Polymerase (blue cap)
8 units/μl Bst DNA Polymerase in 10 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1 % Triton X 100, 50 % (v/v) Glycerol, pH 7.5 (25 °C).
Bst 2.0 Turbo Buffer (red cap) - 10x conc.
200 mM Tris-HCl pH 8.8, 1 M KCl, 100 mM (NH4)2SO4, 60 mM MgSO4, stabilizers and detergents.
MgSO4 Stock Solution (yellow cap)
25 mM MgSO4.
Assay design
Isothermal amplification is an extremely sensitive detection method and care should be taken to avoid contamination of setup areas and equipment with DNA of previous reactions. A common problem is amplification in no-template controls due to:
1. carry-over contamination or
2. amplification of unspecifically annealed primers or primer dimer formations.
As sensitivity and non-template amplification of in-silico designed primers may vary, the evaluation of 2-4 real primer sets before choosing a final set is recommended.