DDAO Phosphate

For general laboratory use.

Envio: shipped at ambient temperature

Condições de armazenamento: store at -20 °C
store dark

Validade: 12 months after date of delivery

Fórmula molecular: C15H12Cl2NO5P (free acid)

Peso molecular: 388.14 g/mol (free acid)

CAS#: 500883-59-0

Pureza: > 95 % (HPLC)

Forma: solid

Propriedades espectroscópicas: λexc 477 nm, λem 628 nm, ε 26.0 L mmol-1 cm-1 (50 mM Potassium Phosphate pH 7.0)

Descrição:
DDAO-phosphate is a long-wavelength, phosphatase substrate for both solution-based assays[1,2] and the amplified detection of specific targets on blots[3,4]. Hydrolysis of the phosphate bond yields a brightly red-fluorescent product with absorption/emission maxima of ~646/659 nm that is efficiently excited by the 633 nm spectral line of the He–Ne laser. The shift of emission maxima between DDAO-phosphate and the dephosphorylated product is larger than 200 nm and permits both species to be easily distinguished. DDAO phosphate has good water solubility, a low KM and a high turnover rate.

Referências selecionadas:
[1] Leira et al. (2000) Characterization of 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl)-phosphate (DDAO) as substrate of PP-2A in a fluorimetric micro-plate assay for diarrhetic shellfish toxins (DSP). Toxicon 38:1833.
[2] Stratis-Cullum et al. (2003) A miniature biochip system for detection of aerosolized Bacillus globigii spores. Anal. Chem. 75:275.
[3] Pretty et al. (2001) Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes. Electrophoresis 22:896.
[4] Kurien et al. (2015) Other notable methods of membrane protein detection: A brief review. Detection of Blotted Proteins, Springer Chapter 36:357.