LEXSY Plating Kit core

For general laboratory use. Not intended for human or animal diagnostic or therapeutic uses.

Envio: Shipped at ambient temperature/on gel packs

Condições de armazenamento: store components as indicated on individual labels

Validade: 12 months

Descrição:
Core kit for preparation of solidified medium for clonal selection of Leishmania tarentolae. Plasticware to be provides by customer.

Contente:
2 x 250 ml of Agar base400 ml of buffered liquid medium:
This component is provided in excess to allow for individual batches.2 x 50 ml heat-inactivated fetal calf serum (iFCS)2 ml of Hemin stock solution, 500x Cat.-No. ML-108S:
0,25% solution of Hemin in 30% Triethanolamine, sterile5 ml of Pen-Strep stock solution, 200x Cat.-No. ML-105S:
10.000 units of penicillin (base) and 10.000 μg of streptomycin (base)/ml as penicillin G sodium salt and streptomycin sulfate in 0.85% saline, sterile

All components are provided sterile.

This kit does not contain nitrocellulose membranes, spatula, dishes, pipettes which are provided with the LEXSY Plating Kit comfort (Cat.-No. ML-451).

Storage conditions: Agar base:
Store at room temperature
Stable for 12 monthsBuffered liquid medium:
Store at 4 °C
Stable for 12 monthiFCS:
Store < -20 °C
Stable for 12 monthHemin stock solution, 500x:
Store at 4 °C in the dark
Stable for 12 monthPen-Strep stock solution, 200x:
Store at -20 °C
Stable for 12 month


Preparation of 500 ml LEXSY plating medium (20 plates, each 25 ml) :Microwave-melt 250 ml Agar base in provided glass flask and keep at 55 °C.Add pre-warmed (37 °C) buffered liquid medium to ca. 400 ml.Add 50 ml iFCS, 1 ml Hemin stock and 2.5 ml Pen-Strep.Fill with buffered liquid medium to 500 ml.Add appropriate antibiotics (not provided).Pour plates with 25 ml pre-warmed (55 °C) serological pipette, avoid air bubbles.After solidification, dry the plates open under the laminar flow box for exactly 10 min, than store them closed bottom-up.After plating, seal plates with parafilm and incubate bottom- up for 5 - 9 days at 26 °C until colonies appear.

Prepare the plates freshly on the day of plating!