Nucleotídeos contendo Mercúrio

The most simple method currently used for solution of the macromolecular phase problem, single wavelength anomalous dispersion (SAD), still involves the incorporation of heavy atoms into protein crystals. After crystallization, finding such derivatives is the second major bottleneck in the determination of the 3D structure of bio-macromolecules.

Most labeling procedures focus on the protein itself in a "trial and error" fashion.
Mercurated C/UTP nucleotides provide an alternative method that allows rational incorporation of heavy atoms into a large number of physiologically relevant enzymes like DNA polymerases and nucleotidyl transferases. Mercury displays a strong anomalous signal at its LIII absorption edge suitable for SAD and MAD experiments.

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