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  3. Click Chemistry
  4. Reagentes Click por Uso
  5. ... em Bactéria/Patógenos
  6. Bactéria Gram-Negativa

Reagentes Click por Uso em Bactéria Gram-Negativa

Gram-negative bacteria are characterized by a lipopolysaccharide-containing outer cell membrane with 3-Deoxy-D-manno-octulosonic acid (KDO) as an essential component. The Azide-functionalized 3-Deoxy-D-manno-octulosonic acid (KDO-Azide) provides an attractive approach for the detection of gram-negative bacteria in living cultures[1]: It is cell-permeable, intracellularly processed and incorporated instead of its natural counterpart KDO.

Tested bacterial strains[1]:
Legionella pneumophila, Salmonella typhimurium, Escherichia coli O86, Escherichia coli K12

Produtos

CuAAC Cell Reaction Buffer Kit (THPTA based) CLK-074

 

CuAAC Cell Reaction Buffer Kit (BTTAA based) CLK-073

 

CuAAC Reaction Ligand Test Kit (THPTA & BTTAA based) CLK-075

 

References

[1] Dumont et al. (2012) Click-Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core. Angew. Chem. Int. Ed. 51:3143.